Complete Amino Acid Sequence of Chitinase-a from Bulbs of Gladiolus (Gladiolus gandavensis)
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منابع مشابه
Complete amino acid sequence of chitinase-a from bulbs of gladiolus (Gladiolus gandavensis).
The complete amino acid sequence of gladiolus bulb chitinase-a (GBC-a) was determined. First the tryptic peptides from GBC-a after it was reduced and S-carboxymethylated were sequenced and then the peptides were further studied by chemical cleavage of the enzyme. GBC-a consisted of 274 amino acid residues and had a molecular mass of 30,714 Da. Two consensus sequences essential for chitinase act...
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compositiens and N-terminal sequences. The number of half-cystine residues in GBC-a and GBC-b was on]y one each, which is much lo"'er than those of plant class l (15--17 Cys residueslmol), c]ass II (S-8Cysresiduesfmol),andc)assEII(6Cysresiduestmol)chitinases. The N-terminal sequenees ot' GBC-a and GBC-b were complete}y different from those ef plant three classes of chitinases. The optimal pHs o...
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peptides were put in order. Of seyen cysteine residues, six were linked by disulfide bonds (between Cys25 alld Cys74, Cys89 and Cys98, and Cys195 and Cys208); Cys176 was free. The enzyme consisted of 208 amino acid residues and had a molecular weight of 22,391. It consisted of only one polypeptide chain withellt a chitin-binding domai". The length of the chain was almost the same as that of the...
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The complete amino acid sequence of pokeweed leaf chitinase-B (PLC-B) has been determined by first sequencing all 19 try, ptic peptides deriyed from the redu ¢ ed and S-carboxy, methylated (RCm-) PLC-B and then connecting them by analyzing the chymotryptic peptides from three fragments produced by cyanogen bromide cleavage of RCm-PLC-B. PLC-B consists of 274 amino acid residues and has a molecu...
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ژورنال
عنوان ژورنال: Bioscience, Biotechnology, and Biochemistry
سال: 1998
ISSN: 0916-8451,1347-6947
DOI: 10.1271/bbb.62.386